Andrographolide Stimulates Neurogenesis in the Adult Hippocampus

Figure 7

ANDRO increases NeuroD1 in the hippocampus of APPswe/PSEN1ΔE9. Seven-month-old APPswe/PSEN1ΔE9 mice were injected i.p. with 2 mg kg⁻¹ ANDRO or vehicle as control 3 times a week per 4 weeks. (a) Immunoblots of β-catenin, inactive form of GSK-3β (phosphorylated in serine-9, pSer9), total GSK-3β, NeuroD1, and β-actin in total protein extracts obtained from the hippocampus of control and ANDRO-treated APPswe/PSEN1ΔE9 mice. (b) Densitometric analysis of β-catenin and GSK-3β pSer9 normalized to β-tubulin and total GSK-3β, respectively. (c) Densitometric analysis of NeuroD1 normalized to β-tubulin. Bars represent mean ± S.E. ( mice). , , and , Student’s -test.

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