Research Article

CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation

Figure 1

CRMP2 and CRMP4 interact with tubulin and actin. (a) Bacterial recombinant glutathione S-transferase- (GST-) CRMPs were purified and subjected to GST-pulldown assays with growth cone extracts from rat brain. Coomassie blue staining of GST and GST-CRMPs (bottom) showed equal loading of bait proteins. The pulldown sediments were subjected to western blot assays with tubulin and actin antibodies using GAPDH as the lysate input control. Each result is representative of three to five separate experiments with similar results. (b) Growth cone lysates from rat brain were subjected to coimmunoprecipitation (Co-IP) with tubulin or actin antibody and then processed for western blot (WB) analysis with the indicated antibodies. (c) Growth cones lysates were immunoprecipitated with CRMP2 or CRMP4 antibodies and processed for WB assays to detect the indicated proteins using the appropriate antibodies.