Research Article

A Novel Nonsense Mutation of POU4F3 Gene Causes Autosomal Dominant Hearing Loss

Figure 4

Immunofluorescence analysis after transient transfection in HEK293 and HEI-OC1 cell lines. Images display DAPI in blue, FLAG-tagged protein in green, and merged pictures. (a) In HEK293 cells, mutant protein located mostly in cytoplasm while the wild-type protein was exclusively located in nuclei. (b) Similar results were observed in HEI-OC1 cells.
(a) HEK293
(b) HEI-OC1