TRPV1 and PLC Participate in Histamine H4 Receptor-Induced Itch

Figure 5

The effects of TRPV1 antagonist capsazepine on the immepip-induced increase in of DRG neurons. (a) H4 agonist immepip (50 μM) and TRPV1 agonist capsaicin (1 μM)-evoked increase in were evidently inhibited by TRPV1 antagonist capsazepine (1 μM) (), after washout of capsazepine, the DRG neuron recovery response to immepip and capsaicin. (c) Immepip (50 μM) and TRPV1 agonist capsaicin (1 μM)-evoked increase in were also obviously blocked by TRPV1 typical antagonist AMG9810 (5 μM) (). (b, d, and e) Normalized Ca²⁺ fluorescence intensity (%) of the responsive neurons in the different blockers (, ).

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