Plastic Change along the Intact Crossed Pathway in Acute Phase of Cerebral Ischemia Revealed by Optical Intrinsic Signal Imaging

Figure 1

Experimental protocols. In Stroke group, the cranial imaging windows were prepared 12 h before the stroke surgery. Optical intrinsic signal (OIS) images were recorded for assessing the somatosensory response at different stages of the experiment (baseline before stroke, and 0 h, 6 h, 12 h, 24 h, and 48 h after stroke). Histologic analysis was performed to confirm the lesion after the experiment. Different from Stroke group, the two Control groups underwent sham surgery instead of photothrombotic stroke. Considering the potential influence of the Rose Bengal or laser illumination, we included two Control groups (Control-RB and Control-Saline). Control-RB rats were illuminated by nonresponsive red laser ( nm, 20 mW), while Control-Saline rats were injected with the same dosage of saline.