Plastic Change along the Intact Crossed Pathway in Acute Phase of Cerebral Ischemia Revealed by Optical Intrinsic Signal Imaging

Figure 3

Schematic of optical intrinsic signal (OIS) imaging system and hindlimb electrical stimulation protocol. (a) The head of rat was fixed in a stereotaxic frame and then illuminated by a 590 nm LED throughout the experiment. The ipsilesional hindlimb was stimulated with different intensity of currents (2 mA, 3.5 mA, and 5 mA). OIS images were captured by a CCD camera connected with a lens and then transferred to the computer for further processing. (b) Each trial of OIS recordings consisted of a 4 s resting state and a 2 s electrical stimulation followed by a 14 s poststimulation state. For each intensity of stimulation, 12 trials of data were recorded at an intertrial interval of 75 s.

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