Anorexia Reduces GFAP+ Cell Density in the Rat Hippocampus

Figure 2

Effect of dehydration-induced anorexia (DIA) on astrocyte density of hippocampal region CA1. Coronal sections of the hippocampus show nuclei labeled with DAPI (a) or immunostained with GFAP (b). The overlay of DAPI and GFAP is shown for control, dehydration-induced anorexia (DIA), and forced food restricted (FFR) experimental groups (c). The densities of nuclei or GFAP+ cells as well as the ratio of astrocyte/nuclei did not differ significantly among experimental groups (; ; for the control, DIA, and FFR groups, respectively; see Table 1). Nuclear and astrocyte densities were estimated in the stratum oriens (s.o.) and stratum radiatum (s.r.); stratum pyramidale (s.p.) is indicated as a reference. Scale bar = 50 μm. Data are mean ± SEM.

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