Anorexia Reduces GFAP+ Cell Density in the Rat Hippocampus

Figure 5

Effect of dehydration-induced anorexia (DIA) on astrocyte density of hippocampal dentate gyrus (DG). Coronal sections of the hippocampus show DAPI (a) or GFAP immunostaining (b). The overlay of DAPI and GFAP is also shown for the control, DIA, and forced food-restricted (FFR) groups (c). The density of nuclei did not differ significantly among experimental groups (). However, a significant reduction was observed for GFAP+ cell density and the astrocyte/nuclei ratio ( and , resp.) (see Table 1). Nuclear and astrocyte densities were estimated in the hilus; subgranular zone (SGZ) and granular cell layer (GCL) are indicated as an anatomical reference. Scale bar = 50 μm. Data are expressed as mean ± SEM.

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