Reorganization of Synaptic Connections and Perineuronal Nets in the Deep Cerebellar Nuclei of Purkinje Cell Degeneration Mutant Mice

Figure 3

Quantification of GAD65/67 and vGlut in DCN. (a) Quantification shows the distribution of GABAergic terminals in different distances from the PN-bearing neurons. The total number of boutons are reduced in pcd compared to wt, regardless of the distance. (b) Somatic glutamatergic terminals at DCN neurons appear to be enhanced in pcd mice. The peripheral synapses remain unaffected by the insult. (c) Western blot analyses of GAD65/67 and vGlut1 with protein extracts of DCN sections. Typical specific bands are visible in both genotypes. Quantification of these bands reveals slight but no significant differences between wt and pcd (GAD65/67 ; vGlut1 ). Data are given as mean ± SEM.

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