Transplantation of Neural Stem Cells Cotreated with Thyroid Hormone and GDNF Gene Induces Neuroprotection in Rats of Chronic Experimental Allergic Encephalomyelitis

Figure 4

Immunohistochemical double staining of BrdU, MAP2, GFAP, and GalC positive cells in the cerebral parenchyma of rats. (a–c) MAP2⁻/BrdU⁺ cells (solid arrow), MAP2⁺/BrdU⁻ cells (dotted arrow), and MAP2⁺/BrdU⁺ cells (arrowhead) in NSCs, T3/NSCs, and GDNF-T3/NSCs group, respectively. (d–f) GalC⁻/BrdU⁺ positive cells (solid arrow), GalC⁺/BrdU⁻ cells (dotted arrow), and GalC⁺/BrdU⁺ cells (arrowhead) in NSCs, T3/NSCs, and GDNF-T3/NSCs group, respectively. (g–i) GFAP⁻/BrdU⁺ cells (solid arrow), GFAP⁺/BrdU⁻ cells (dotted arrow), and GFAP⁺/BrdU⁺ cells (arrowhead) in NSCs, T3/NSCs, and GDNF-T3/NSCs group, respectively. (j) GFAP⁺ cells (dotted arrow) in control group. (k) GFAP⁺ cells (dotted arrow) in normal group. Bar (a–k) = 25 μm.

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