A Comparative Analysis of the Endocannabinoid System in the Retina of Mice, Tree Shrews, and Monkeys

Figure 6

NAPE-PLD immunoreactivity in rod photoreceptors. Vertical sections from the mouse retina (first column), tree shrew retina (second column), vervet retina (third column), and macaque retina (fourth column). Confocal micrographs of coimmunolabeling for the synthesizing enzyme NAPE-PLD and the cell-type-specific marker for rods, rhodopsin. Each immunofluorescent signal is presented alone in grayscale: NAPE-PLD in the first line and rhodopsin in the second line; then the two are presented merged (third line: NAPE-PLD in magenta and rhodopsin in green). Arrowheads point to rhodopsin-positive cell bodies that express NAPE-PLD in vervet and macaque monkeys only, and arrows mark the lack of colocalization. ONL: outer nuclear layer. Scale bar = 30 μm.