A Comparative Analysis of the Endocannabinoid System in the Retina of Mice, Tree Shrews, and Monkeys

Table 1

List of antibodies used in this study.

Antibody^1	Immunogen	Source^2	Working dilution
CB	Purified bovine kidney calbindin-D28K	Sigma-Aldrich, St. Louis, MO; C9848, mouse monoclonal, clone CB0955	1 : 250
CB1R	Fusion protein containing aa 1–77 of rat; CB1R	Calbiochem, Gibbstown, NJ; 209550, rabbit polyclonal	1 : 150
CB2R	Synthetic peptide corresponding to aa; 20–33 of human CB2R	Cayman Chemical, Ann Arbour, MI; 101550, rabbit polyclonal	1 : 150
DAGL	Peptide with sequence CPAKQDELVISAR, from the C Terminus of the protein sequence	Novus, Littleton, CO; NBP2-31856, rabbit polyclonal	1 : 100
FAAH	Synthetic peptide aa 561–579 of rat FAAH	Cayman Chemical, Ann Arbour, MI; 101600, rabbit polyclonal	1 : 150
GS	Full protein purified from sheep brain	Chemicon, Temecula, CA; MAB302,; mouse monoclonal, clone GS-6	1 : 500
MAGL	Human MAGL aa 1–14	Cayman Chemical, Ann Arbour, MI; 100035, rabbit polyclonal	1 : 150
NAPE-PLD	Purified protein corresponding to aa 159–172 NAPE-PLD human	Cayman Chemical, Ann Arbour, MI; 10305, rabbit polyclonal	1 : 200
Rhodopsin	Bovine rhodopsin	Abcam, Toronto, ON; ab98887, mouse monoclonal, clone Rho 4D2	1 : 500
PKC	Peptide mapping the aa 296–317 of; human PKCα	Santa Cruz Biotechnology, Santa Cruz,; CA; sc-8393, mouse monoclonal,; clone H-7	1 : 500

CB: calbindin; CB1R: cannabinoid receptor type 1; CB2R: cannabinoid receptor type 2; DAGL: diacylglycerol lipase alpha; FAAH: fatty acid amide hydrolase; GS: glutamine synthetase; MAGL: monoacylglycerol lipase; NAPE-PLD: N-acyl phosphatidylethanolamine-specific phospholipase D; PKC: protein kinase C (alpha isoform); aa: amino acids.
²The source column indicates the commercial company, catalog reference, and origin. The clone designation is given for monoclonal antibodies.