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Neural Plasticity
Volume 2016, Article ID 3497901, 13 pages
http://dx.doi.org/10.1155/2016/3497901
Research Article

Increase in Growth Cone Size Correlates with Decrease in Neurite Growth Rate

1Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
2Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USA
3Purdue Institute for Integrative Neuroscience, Purdue University, West Lafayette, IN 47907, USA

Received 3 February 2016; Accepted 3 April 2016

Academic Editor: Rajnish Chaturvedi

Copyright © 2016 Yuan Ren and Daniel M. Suter. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Video 1. DIC time-lapse imaging of Aplysia growth cones on PLL-coated coverslip 48 h after plating. C domain in the large growth cone (left) swayed and split, while the P domain remained largely stationary except for small scale protrusions and retractions. In contrast, the small growth cone (right) advanced at the same time. Scale bar and time as indicated. Time compression: 1200x. Data for Figure 4a was taken from this video.

Video 2. DIC time-lapse imaging of Aplysia growth cone on PLL-coated coverslip 53 h after plating. A smaller growth cone showed persistent advancement of about 20 μm in 3 h. Scale bar and time as indicated. Time compression: 1200x. Data for Figure 4b was taken from this video.

Video 3. DIC time-lapse imaging of two Aplysia growth cones on PLL-coated coverslip immediately after plating and followed for 12 h. The formation of extensive lamellipodia coincided with cessation of motility of left growth cone 1 (GC1 in Figure 5a). At 7 h, the stage was repositioned. The right growth cone 2 (GC2) resumed rapid advance at 9 h. Scale bar and time as indicated. Time compression: 5250x. Data for Figure 5a was taken from this video.

Video 4. DIC time-lapse imaging of Aplysia growth cone at the interface of PLL-coated coverslip and collagen I gel. Growth cone was compact and small, exhibited more filopodia than lamellipodia, and showed persistent advancement of about 20 μm in 3 h. Scale bar and time as indicated. Time compression: 1200x. Data for Figure 6 was taken from this video.

  1. Supplementary Material