Increases in Retrograde Injury Signaling Complex-Related Transcripts in Central Axons following Injury

Figure 1

Hippocampal explant injury model. (a) Schematic of axonal injury, performed 2/3 distance away from the edge of the explant. Axons were collected proximal and distal to the injury site. Cell bodies (shaded red) were excluded from collection. (b) Bright-field image illustrating long, narrow axons before and after axonal injury. (c) Double-label immunofluorescence of SMI-31 (red) and MAP2 (green) showed robust axonal outgrowth and very few or no dendrites in the same region (green nonspecific binding). Arrow indicates direction of outgrowth. Asterisk () indicates explant mass. Additional details of explant system are provided in [20]. Bars are 75 μm.

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