The Response to Oxidative DNA Damage in Neurons: Mechanisms and Disease

Figure 4

Levels of BER enzymes in neurons derived from cortex (CX), hippocampus (HP), and cerebellum (CV). Immunoblotting was carried out by using antibodies specific for DNA ligase 3 (103 kDa, BD Biosciences Pharmingen, San Diego, CA), FEN1 (43 kDa, Abcam, Atlanta, USA), XRCC1 (85 kDa, Bethyl Laboratories, Inc.), APE1 (35.5 kDa, Santa Cruz Biotechnology, Inc.), and DNA Pol (38 kDa, Trevigen Inc., Gaithersburg, MD). -actin (40 kDa, Sigma) and α-tubulin (55 kDa) were used as loading controls. Western blot analysis was conducted on 15 μg of whole cell extracts. One representative experiment is shown.