Figure 2: DAMGO-dependent modulation of the embryonic respiratory rhythm. (a) Schematic of an embryonic transverse brainstem slice used for extracellular electrode recording from the isolated preBötC network. Integrated network activity (Int preBötC) recorded in control conditions (top), in the presence of 0.25 μM DAMGO (middle) and after 30 minutes’ wash-out (bottom). (b) Pooled data showing mean (± SEM) inspiratory burst frequency obtained from 10 embryonic slices in control conditions (CTL, white bar), after 15 min exposure to 0.25 μM DAMGO (black bar) and after wash-out (gray bar). DAMGO significantly decreased the preBötC rhythm frequency. This effect was reversible. Asterisk indicates .