Mechanisms Underlying Adaptation of Respiratory Network Activity to Modulatory Stimuli in the Mouse Embryo

Figure 6

The embryonic respiratory network and respiratory pacemaker neurons respond to temperature changes. (a) Left: schematic of an embryonic transverse slice used for extracellular electrode recording from the isolated preBötC network. Middle: integrated traces of spontaneous network activity recorded at 28°C (left) and at 32°C (right). Right: pooled data showing mean inspiratory burst frequency (± SEM) in 7 slices in control conditions at 28°C (white bar) and at 32°C (black bar). Asterisk: . (b) Left: fluorescent image showing location of 4 preBötC network neurons monitored with calcium imaging. Middle: simultaneous recordings of network electrophysiological activity (Int preBötC) and calcium transients in the 4 individual cells in control conditions at 28°C (left), after synaptic isolation in cocktail at 28°C (middle), and in cocktail at 32°C (right). Note that the frequency of spontaneous cyclic activity in isolated cells 1 and 3 increased at 32°C, while that of cell 4 decreased and cell 2 only became active at the elevated temperature. Right: pooled data showing mean frequency (± SEM) of activity expressed by 14 pacemaker neurons under cocktail at 28°C (white bar) and in cocktail at 32°C (black bar). Asterisk: .

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