Protective Effect of Edaravone on Glutamate-Induced Neurotoxicity in Spiral Ganglion Neurons

Figure 6

Cytoprotection of edaravone on glutamate-induced apoptosis and necrosis in SGNs. (a) Untreated SGNs appeared blue intact nuclei. (b) SGNs were treated with glutamate for 10 min and then cultured for 24 h. Nuclei of apoptotic SGNs were obvious and stained brilliant-blue by Ho.33342. Nuclei of necrotic cells were labeled red by PI. (c) SGNs were pretreated with 500 μM edaravone 20 min before glutamate. (d and e) Apoptosis and necrosis rates of SGNs. , versus glutamate-treated group.

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