Amyloid-Beta Induced Changes in Vesicular Transport of BDNF in Hippocampal Neurons

Figure 3

A subpopulation of BDNF-containing vesicles colocalize with APP. (a) Dissociated hippocampal cultures of 5xFAD mice and wild-type littermates were transfected with BDNF-GFP (green) and immunostained with an antibody directed against the N-terminus of endogenous APP (red). Representative pictures showing a transfected and immunostained hippocampal WT neuron (upper part) and 5xFAD neuron (lower part) and higher magnification of boxed areas. Mature BDNF colocalized with APP in vesicular structures. (b) Percentage of αAPP staining colocalizing with total BDNF-GFP area. Error bars represent SEM.

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