The Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons

Figure 4

activation by Mas-7 regulates dendritic spine morphogenesis. (a) Left panel, representative images of 14 DIV hippocampal neurons treated with Mas-7 for 0, 30, or 120 min. Right panel, 3D reconstructions of neurites. Scale bar = 5 μm. (b) Quantification of dendritic spine density. (c) Quantification of spine head width. (d) Quantification of spine length (). , , and . (e) Mas-7 induces de novo formation of dendritic protrusions. Live cell time-lapse imaging of the formation of dendritic spines in response to Mas-7 in 14 DIV hippocampal neurons. A dendrite of an EGFP-transfected neuron is shown before and after 5, 30, and 40 min of treatment with Mas-7. Scale bar = 2 μm.

(a)  

(b)

(c)

(d)

(e)