The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro

Figure 3

3D culture protected SGN explants from apoptosis. (a) The diagram of the assay. (b) Apoptotic SGNs (white arrow) were observed in 2D culture, characterized by DAPI (grey), TUNEL (green), and β-tubulin (red) costained. (c) Compared with 2D culture, 3D culture reduced the average number of apoptotic SGNs. . (d) 3D culture regulated the mRNA expressions of apoptotic genes. and .

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