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Figure 5: 3D culture affected the growth of growth cones. (a) The diagram of the assay. (b) Morphology of the SGNs growth cone cultured in 2D and 3D systems. Phalloidin, green; β-tubulin, red. (c) Quantification of the average number of filopodia per growth cone. (d) Quantification of the average length of filopodia from the edge of the growth cone to tips of each filopodia. (e) Quantification of the average area per growth cone. and .