The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro

Figure 7

3D culture elevated synapse density in SGN explants. (a) The diagram of the assay. (b) Representative images of SGNs explants immunostained with neurofilament (green) and synaptophysin (red) cultured in 2D and 3D systems. (c) Representative images in high magnification of synaptophysin (red) immunostained SGN explants in 2D and 3D systems. (d) Quantification of the number of synapse puncta in 2D and 3D systems. .

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