Programming of Dopaminergic Neurons by Neonatal Sex Hormone Exposure: Effects on Dopamine Content and Tyrosine Hydroxylase Expression in Adult Male Rats

Figure 5

Dopamine release in nucleus accumbens (NAcc) of adult male with neonatal exposure to testosterone propionate (TP), dihydrotestosterone (DHT), or estradiol valerate (EV). Panel (a) (left) shows a typical placement of microdialysis probe in NAcc. Panel (a) (right) shows a scheme of NAcc extracted from the rat brain atlas [41] with an example of the theoretical probe position. Panel (b) shows extracellular dopamine levels in the NAcc and the grey bar indicates the time during which Krebs-Ringer’s phosphate (KRP) buffer-K⁺ [70 mM] was perfused. Asterisk indicates a significant difference when comparing the effect of KRP-K⁺ between the respective experimental groups (; one-way ANOVA followed by the Newman-Keuls multiple comparison test). Results are expressed as percentage of the respective basal levels (mean) ± SEM ( for male control and for each treatment group). MS, medial septum; LSI, lateral septum intermediate; LSV, lateral septum ventral; LSD, lateral septum dorsal; cc, corpus callosum; LV, lateral ventricle; CPu, caudate putamen.

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