Inhibition of DNA Methylation Impairs Synaptic Plasticity during an Early Time Window in Rats

Figure 7

The phosphorylation of MeCP2 at S80 and S421 at two hours after tetanic stimulation in the presence of 5AZA. (a) Representative immunostaining for the neuronal marker anti-β-tubulin III (green) and Hoechst 33342 (blue) in the hippocampal CA1 region. Calibration bar, 50 μm. (b) Quantification of the phosphorylation of MeCP2 at S80 at vehicle (number of nuclei = 62) and tetanized (number of nuclei = 98) slices at two hours after tetanic stimulation in the presence of 5AZA. , using Student’s -test. (c) Quantification of the phosphorylation of MeCP2 at S421 at vehicle (number of nuclei = 136) and tetanized (number of nuclei = 164) slices at two hours after tetanic stimulation in the presence of 5AZA. , using Student’s -test.

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