Dose-Dependent Differential Effect of Neurotrophic Factors on In Vitro and In Vivo Regeneration of Motor and Sensory Neurons

Figure 1

Representative images of RT97 stained neurites from spinal cord slices (a–c) and DRG neurons (d–f) cultured within a 3D collagen matrix alone (a, d) and with addition of 500 ng/mL of FGF-2 (b, e) or 100 ng/mL of GDNF (c, f). Quantification of the number of neurites grown at increasing distance from the spinal cord slices (g, h) and from DRG body (k, l) after the addition of FGF-2 and GDNF. Plots of the quantified AUC from (g), (h), (k), and (l) graphs for motor (i, j) or sensory (m, n) neurite outgrowth. Data expressed as mean ± SEM. versus control; . Scale bar: 100 μm (a) and 200 μm (b–f).