Dose-Dependent Differential Effect of Neurotrophic Factors on In Vitro and In Vivo Regeneration of Motor and Sensory Neurons

Figure 3

Representative images of RT97 stained neurites from spinal cord slices (a–c) and DRG neurons (d–f) cultured within a 3D collagen matrix alone (a, d) and with addition of 10 ng/mL (b, e) and 50 ng/mL of BDNF (c, f). Quantification of the number of neurites grown at increasing distance from the cord slices (g) and from DRG body (i) after the addition of different doses of BDNF. Plots of the quantified area under each curve from (g) and (i) graphs for motor (h) or sensory neurite outgrowth (j). Data expressed as mean ± SEM. versus control; . Scale bar: 200 μm (a–f).