Effects of Microtubule Stabilization by Epothilone B Depend on the Type and Age of Neurons

Figure 5

Epothilone B induces changes in growth cone MT structure. (a-b) Embryonic (E15.5) cortical neurons, embryonic (E13.5) DRG neurons, or adult DRG neurons were cultured in the absence or presence of epothilone B (either 10 pM or 1 nM) and were fixed, followed by immunostaining with anti-Tuj1 antibodies. Exposure time has been adjusted for each image to optimize the visibility of the MT network. Representative images (a) and quantifications of growth cone MT network (b) are shown. Scale bar, 10 μm. < 0.05, statistically not significant.