Effects of Microtubule Stabilization by Epothilone B Depend on the Type and Age of Neurons

Figure 7

Nocodazole-induced degeneration in different types of neurons. (a–c) Embryonic (E15.5) cortical neurons, embryonic (E13.5) DRG neurons, or adult DRG neurons were cultured in the absence or presence of nocodazole (Ncd) (1 μM, 30 min), as indicated. Neurons were immunostained with anti-Tuj1 antibodies. Representative images (a) and quantification of axon degeneration (b) and soma diameter (c) are shown. Scale bar, 50 μm. < 0.05 and < 0.001; ns, statistically not significant.