MicroRNA-132 Interact with p250GAP/Cdc42 Pathway in the Hippocampal Neuronal Culture Model of Acquired Epilepsy and Associated with Epileptogenesis Process

Figure 2

Knockdown of miR-132 and p250GAP in cultured neurons. (a) Cells were transfected with ant-132 at DIV7 and observed using a fluorescence microscope 48 h after transfection. Transfection efficiency was measured by RFP fluorescence. (b) Cells were transfected with LV-shp250GAP at DIV7 and observed 72 h after transfection. Transfection efficiency was measured by GFP fluorescence. Scale bar is 50 µm. (c, d) Measurement of knocked down expression in transfected neurons was performed by qRT-PCR for miR-132 and by WB for p250GAP. (e) Expression level of p250GAP 72 h after ant-132 treatment in cultured epileptic hippocampal neurons. (; the data are representative of 5 experiments).

(a)  

(b)

(c)

(d)

(e)