MicroRNA-132 Interact with p250GAP/Cdc42 Pathway in the Hippocampal Neuronal Culture Model of Acquired Epilepsy and Associated with Epileptogenesis Process

Figure 3

(a1-a2) The levels of active Cdc42 and Rac1 were significantly elevated in MGF-treated neurons compared to control neurons. (b1-b2) Effect of ant-132 and LV-shp250GAP transfection on active Rac1 levels. Cultured hippocampal neurons were transfected at DIV7 with ant-132 and LV-shp250GAP, and the level of active Rac1 was detected at DIV13. Transfection of ant-132 or LV-shp250GAP did not significantly affect Rac1 activity. (c1-c2) Cdc42 activation was significantly inhibited after ant-132 transfection and elevated after LV-shp250GAP transfection. (d1-d2) Effect of ant-132 and LV-shp250GAP transfection on Rac1 activation level in the MGF medium-treated hippocampal neurons. Cultured hippocampal neurons were transfected at DIV7 with ant-132 and LV-shp250GAP and then treated with MGF medium at DIV10. The level of active Rac1 was detected at DIV13. Rac1 activation level in the MGF medium-treated hippocampal neurons was not affected by ant-132 and LV-shp250GAP. (e1-e2) The activation level of Cdc42 in the MGF medium-treated hippocampal neurons was significantly affected by the inhibition of miR-132 and p250GAP expression. ; (; the data are representative of 5–7 experiments).