Blockade of Toll-Like Receptors (TLR2, TLR4) Attenuates Pain and Potentiates Buprenorphine Analgesia in a Rat Neuropathic Pain Model

Figure 5

The influence of once daily, repeated intrathecal administration of vehicle (“V”, sterile water), LPS-RS [20 μg/5 μL], or LPS-RS Ultrapure [“LPS-RSU’’, 20 μg/5 μL] on pain behavior, as measured by von Frey’s test (mechanical allodynia; (a), (b)) and the cold plate test (thermal hyperalgesia; (c), (d)), 1 and 3 hours after drug administration on 2nd and 7th days after chronic constriction injury (CCI) to the sciatic nerve. The data are presented as the mean ± SEM (10–25 rats per group). Intergroup differences were analyzed using one-way ANOVA followed by Bonferroni’s multiple comparisons test. , , and indicate significant differences compared with the vehicle-treated, CCI-exposed rat group (V-CCI).