Blockade of Toll-Like Receptors (TLR2, TLR4) Attenuates Pain and Potentiates Buprenorphine Analgesia in a Rat Neuropathic Pain Model

Table 1

Western blot analysis of IBA-1, TLR2, TLR4, MyD88, and TRIF protein levels in the contralateral dorsal lumbar spinal cord and DRG tissue on 7th day after chronic constriction injury (CCI) in rats. The results are not statistically significant and are presented as the mean ± SEM of 4–7 samples per group (see also Figure 4).

Protein level	7th day after CCI
	Dorsal lumbar section, contralateral side
	INTACT		Vehicle-CCI
Spinal cord	IBA-1	1.00 ± 0.07	IBA-1	1.12 ± 0.19
	TLR2	1.00 ± 0.13	TLR2	1.17 ± 0.08
	TLR4	1.00 ± 0.06	TLR4	1.11 ± 0.13
	MyD88	1.00 ± 0.05	MyD88	1.09 ± 0.06
	TRIF	1.00 ± 0.04	TRIF	0.99 ± 0.06
DRG	IBA-1	1.00 ± 0.10	IBA-1	1.02 ± 0.08
	TLR2	1.00 ± 0.08	TLR2	0.81 ± 0.11
	TLR4	1.00 ± 0.16	TLR4	0.98 ± 0.07
	MyD88	1.00 ± 0.11	MyD88	0.98 ± 0.05
	TRIF	1.00 ± 0.03	TRIF	1.00 ± 0.08