Inhibition of the cAMP/PKA/CREB Pathway Contributes to the Analgesic Effects of Electroacupuncture in the Anterior Cingulate Cortex in a Rat Pain Memory Model

Figure 7

EA decreased CREB phosphorylation in the ACC of pain memory model rats. The right ACC tissues of different groups were prepared and labeled with immunofluorescence (, five slides for each rat). Total protein extracts from the ACC tissues were detected by western blotting (). (a) Views of p-CREB-positive cells in the ACC (scale bar, 50 μm). (b) Quantification of p-CREB-positive cells showed that EA treatment at first injection time point significantly reduced the amount of positive p-CREB. (c) Western blotting analysis of p-CREB in the different groups. (d) Quantitative analysis using the western blot assay showed that EA significantly reduced phosphorylation level of CREB. Error bars indicated SEM. and , versus the control group; and , versus the model group; and , versus the indo group; , versus the sham EA group.

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