Inhibition of the cAMP/PKA/CREB Pathway Contributes to the Analgesic Effects of Electroacupuncture in the Anterior Cingulate Cortex in a Rat Pain Memory Model

Figure 8

EA inhibited the colocalization of cAMP with p-PKA in the ACC of pain memory model rats. The right ACC tissues from each group were prepared and labeled with double-immunofluorescence (, five slides for each rat). (a) Views of the colocalization of cAMP with p-PKA (scale bar, 20 µm). (b) The magnified image shows the colocalization of cAMP with p-PKA. (c) Double-labeled immunofluorescence analysis demonstrated that EA significantly reduced the amount of colocalized cAMP and p-PKA. Error bars indicated SEM. , versus the control group; , versus the model group; , versus the indo group; , versus the sham EA group.

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