Regeneration of Zebrafish CNS: Adult Neurogenesis

Figure 3

Analysis of BrdU incorporation and expression of neuronal marker HuC/D in adult zebrafish spinal cord. Longitudinal sections of uninjured (a) and a 7-day crush injured cord (b) immunostained with neuronal marker HuC/D. Neurons (thick arrow) are in the subependyma of uninjured cord and newly generated neurons (thin arrow) in the injury epicenter (yellow arrow) of 7-day injured cord. EPC marks the ependymal canal of the cord. (c) Schematic diagram showing locations of proliferating cells in adult uninjured spinal cord (c) and injured cord (d) after BrdU incorporation study. WM: white matter; GM: grey matter. (e) Quantification of proliferating cells after crush injury in zebrafish spinal cord (adapted from [13]). Scale bar = 200 μm (a, b).

(a) No injury

(b) 7 days after crush injury

(c)

(d)

(e)