Social Isolation Stress Induces Anxious-Depressive-Like Behavior and Alterations of Neuroplasticity-Related Genes in Adult Male Mice
Social isolation stress in adulthood reduces BDNF-7 splice variants in the hippocampus (HPC) and prefrontal cortex (PFC). (a-b) Adult socially isolated mice showed decreased levels of BDNF-7 mRNA transcript in the HPC (a) and PFC (b). On the contrary, total BDNF and BDNF splice variants 1, 2, 3, 4, 6, and 8 were not modulated by adult SI stress. Data are presented as Mean ± SEM (-8 per group). . (c–f) Pro-BDNF and mature BDNF protein levels in the HPC (c, e) and prefrontal cortex (d, f). Densitometric quantifications were obtained as ratio of Pro-BDNF/β-actin and BDNF/β-actin. Data are presented as mean ± SEM ( per group). (e-f) Representative Western blot pictures from Pro-BDNF, BDNF, and β-actin.
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