Gypenoside Attenuates β Amyloid-Induced Inflammation in N9 Microglial Cells via SOCS1 Signaling

Figure 1

Experimental protocol diagram. (a) The N9 cells were divided into three groups; after being treated for 24 h as shown in the figure, western blot analysis and immunocytochemistry were performed to assess iNOS and Arg-1 protein expressions. (b) The cells were divided into four groups, after 24-h incubation, western blot and ELISA were used to evaluate IBA-1 expression and cytokines releases, respectively. (c) The cells were divided into three groups, after being treated as shown in the figure, western blot was used to evaluate the SOCS1-siRNA-induced SOCS1 protein expression. (d) The cells were divided into five groups, after the treatments, iNOS and Arg-1 expressions and cytokines releases were measured.

(a)

(b)

(c)

(d)