Gypenoside Attenuates β Amyloid-Induced Inflammation in N9 Microglial Cells via SOCS1 Signaling

Figure 6

SOCS1-siRNA abolished GP-induced effects on iNOS and Arg-1 expressions and TNF-α and IL-10 releases. Western blot was taken to evaluate the SOCS1-siRNA-induced effect on SOCS1 protein expression ((a), ). Then the cells were divided into five groups, including control: cells cultured in normal medium; Aβ: cells cultured in the medium containing 10 μM Aβ; GP + Aβ: cells cultured in the medium containing 50 μg/mL GP and 10 μM Aβ; SOCS1-siRNA + GP + Aβ: cells treated with SOCS1-siRNA for 6 h and then exposed to the medium containing 50 μg/mL GP and 10 μM Aβ; SC-siRNA + GP + Aβ: cells treated with scrambled (SC)-siRNA for 6 h and then exposed to the medium containing 50 μg/mL GP and 10 μM Aβ. After 24-h incubation, the iNOS and Arg-1 expressions were evaluated by using western blot analysis ((b)-(c), ), and TNF-α and IL-10 concentrations were assessed by using ELISA kits ((d)-(e), ). (f) Hypothetical model of SOCS1 mediates GP-induced anti-Aβ effect by switching microglia from M1 to M2 state. Data are means ± SD, : , NS: no significance.

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