Status Epilepticus Enhances Depotentiation after Fully Established LTP in an NMDAR-Dependent but GluN2B-Independent Manner

Figure 2

LFS-induced DP in epileptic tissue is not GluN2B-dependent. (a, b) Time course of the experiment showing the relative fEPSP slope (in % baseline). The GluN2B blocker Ro 25-6981 was applied as indicated by the bar. While LTP in post-SE tissue (closed symbols) was significantly enhanced as compared to control (open symbols) at timepoint 2 (60 min after TBS), there was no significant difference between both groups at timepoint 3 (i.e., DP) despite the presence of the GluN2B blocker. Sample traces (panel a) were taken at baseline (timepoint 1), directly before LFS (i.e., LTP, timepoint 2), and at the end of the experiment (i.e., DP, timepoint 3). (c) Bar graph summarizing the relative fEPSP slopes (in % baseline) for three different timepoints (baseline, LTP, and DP). Note that timepoints 2 and 3 are in the presence of the GluN2B blocker Ro 25-6981. Diamonds indicate significant differences against baseline. Asterisks indicate significant differences as indicated by the brackets. (d) Paired-pulse ratio (PPR) of synaptic transmission following double-pulse stimulation (interstimulus interval 40 ms) for control (open bars) and post-SE tissue (closed bars) at three timepoints (baseline, LTP, and DP).

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