Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice

Figure 1

CRISPR/Cas9-mediated generation of Myh14 knockout mice. (a) Schematic diagram of sgRNA at Myh14 Exon 9 locus (indicated by the red arrow). The sgRNA sequence is underlined in black, and the PAM sequence is shown in red. (b) Sequencing chromatograms of Myh14^−/− mice. The sequence at the start of the mutated site becomes scrambled. (c) Three types of mutations (3, 6 bp deletions and 1 bp insertion) were produced. Type 3 frameshift mutation was chosen for further analysis. (d) Frameshift mutation of Myh14^−/− mice. The mutation is underlined in black. “” indicates a premature stop codon. The translation of the protein is terminated at the point of the arrow.

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