Drosophila Torsin Protein Regulates Motor Control and Stress Sensitivity and Forms a Complex with Fragile-X Mental Retardation Protein

Figure 4

Reduced expression of DTor impaired locomotor behavior. Flies of different ages expressing DTor-cDNA or DTor-RNAi under control of various Gal4 drivers were monitored in vials at 38°C for 10 min. The number of active flies was plotted at each time point. Asterisks indicate the time points showing a significant difference () from -test analysis. (a) Tub-Gal4 drivers, (b) C155-Gal4 drivers, and (c) C57-Gal4 drivers. Ten to twelve groups of Tub-Gal4/+, UAS-DTor-cDNA/+; Tub-Gal4/+, pWIZ-DTor-RNAi/+; Tub-Gal4/, C155-Gal4, C155-Gal4; UAS-DTor-cDNA/+, C155-Gal4; pWIZ-DTor-RNAi/+, C57-Gal4/+, UAS-DTor-cDNA/+; C57-Gal4/+, and pWIZ-DTor-RNAi/+; C57-Gal4/+ were collected from three independent crosses and examined.

(a) Ubiquitous expression

(b) Pan-neural expression (presynaptic terminals)

(c) Postsynaptic terminal expression