Carbachol-Induced Reduction in the Activity of Adult Male Zebra Finch RA Projection Neurons

Figure 1

Carbachol produced a concentration-dependent decrease in the spontaneous AP firing frequency and induced a concentration-dependent membrane hyperpolarization in RA projection neurons. (a) A representative whole-cell recording showed the reversible effects of 30 μM carbachol on the spontaneous AP firing frequency and membrane hyperpolarization. (A, B, C) Traces from the box area in (a) in expanded time scales. (b) Inhibitory effects of carbachol on the spontaneous AP firing frequency. The number of neurons was 6, 6, 7, 11, 7, and 7 in 0, 10, 20, 30, 40, and 50 μM carbachol, respectively. (c) Hyperpolarization effects of carbachol on the resting membrane potential. The number of neurons was the same to (b). ; , paired test, as compared with 0 μM carbachol group.

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