Neuregulin-1 Regulates Cortical Inhibitory Neuron Dendrite and Synapse Growth through DISC1

Figure 3

NRG1 functions through DISC1 to regulate cortical inhibitory neuron dendrite growth. (a) Representative images of DIV21 cortical inhibitory neurons cotransfected with -GFP and -DISC1FL or -DISC1DN on DIV7. Cells were treated with PBS (top panels) or NRG1β (bottom panels) for 2 days. Cultures were stained for GFP to enhance to GFP signal. Images were acquired at 20x. Scale bar = 100 μm. (b) Dendrite growth was analyzed by Sholl analysis using ImageJ. (c) At baseline conditions (PBS treatment), expression of -DISC1DN resulted in a significant decrease in total dendrite growth compared to -GFP controls; expression of -DISC1FL had no effect compared to -GFP controls. In cells treated with NRG1β, both -DISC1FL and -DISC1DN caused a significant decrease in total dendrite growth compared to -GFP controls. Significance determined using a one-way analysis of variance (ANOVA) with Tukey’s post hoc tests. Error bars represent standard error of the mean, –54 cells per condition from 3 experiments; , , and .

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