Review Article
Endogenous and Synthetic Cannabinoids as Therapeutics in Retinal Disease
Figure 3
Involvement of the CB1 receptor on the neuroprotective actions of HU-210. (a) ChAT immunoreactivity. The selective inverse agonist AM251 attenuated the neuroprotective actions of HU-210 on cholinergic amacrine cells ((A) control, ; (B) chemical ischemia mixture, ; (C) HU-210 (10−5 M), ; (D) HU-210 (10−5 M) + AM251 (10−5 M)). Arrows depict ChAT-immunoreactive amacrine cells. Scale bar: 50 μm. (b) Effect of cannabinoids on retinal cell death. TUNEL staining was prominent in the chemical ischemia incubated tissues ((B) ) while less staining was observed in the control tissues ((A) ). TUNEL staining substantiates the neuroprotective effects of HU-210 ((C) 10−5 M, ) and the reduced neuroprotection (increased TUNEL staining) in the presence of AM251 ((D) 10−5 M, ). ONL: outer nuclear layer; INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer.
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