Plasma Membrane Targeting of Protocadherin 15 Is Regulated by the Golgi-Associated Chaperone Protein PIST

Figure 1

PCDH15-CD3 interacts with PIST. (a) Schematic diagram of PCDH15 and PIST domain structure. EC, extracellular cadherin repeats; TM, transmembrane domain; CR, common region; CC1, coiled-coil domain 1; CC2, coiled-coil domain 2; PDZ, PSD-95/discs large/ZO-1 domain. The C-terminal PBI of PCDH15 isoforms (STSL, NTAL, and MTKL) is also indicated. (b) Western blots showing coimmunoprecipitation (co-IP) of EGFP-tagged mouse PCDH15-CD1 cytoplasmic domain with Myc-tagged human PIST. (c) Western blots showing co-IP of EGFP-tagged mouse PCDH15-CD2 cytoplasmic domain with Myc-tagged human PIST. (d) Western blots showing co-IP of EGFP-tagged mouse PCDH15-CD3 cytoplasmic domain with Myc-tagged human PIST. IP indicates antibody used for immunoprecipitation and WB indicates antibody used for detection.

(a)

(b)

(c)

(d)