Research Article
Plasma Membrane Targeting of Protocadherin 15 Is Regulated by the Golgi-Associated Chaperone Protein PIST
Figure 2
Colocalization of PCDH15-CD3 with PIST in COS-7 cells. (a) In the presence of EGFP-PIST, Myc-PCDH15-CD1 localizes in the cytoplasm as well as on the cell membrane. (b) In the presence of EGFP-PIST, Myc-PCDH15-CD2 localizes in the cytoplasm as well as on the cell membrane. (c) In the presence of EGFP-PIST, Myc-PCDH15-CD3 colocalizes with EGFP-PIST in the TGN. Myc-PCDH15 was stained with mouse anti-Myc antibody and then TRITC-conjugated goat anti-mouse secondary antibody. Nuclei were stained with DAPI. The fluorescent intensity was quantified using Image J. Scale bars, 10 μm.
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