Plasma Membrane Targeting of Protocadherin 15 Is Regulated by the Golgi-Associated Chaperone Protein PIST

Figure 3

The C-terminal PBI of PCDH15-CD3 is necessary for the interaction with PIST. (a) Schematic diagram of PCDH15 and PIST domains used in this experiment. (b) Western blots showing coimmunoprecipitation (co-IP) of EGFP-tagged human PIST PDZ domain with Myc-tagged mouse PCDH15-CD3 cytoplasmic domain. (c) Western blots showing co-IP of EGFP-tagged human PIST CC2-plus region with Myc-tagged mouse PCDH15-CD3 cytoplasmic domain. (d) Western blots showing co-IP of EGFP-tagged human PIST with Myc-tagged mouse PCDH15-CD3 cytoplasmic domain with or without the C-terminal PBI (MTKL). IP indicates antibody used for immunoprecipitation and WB indicates antibody used for detection.

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