Plasma Membrane Targeting of Protocadherin 15 Is Regulated by the Golgi-Associated Chaperone Protein PIST

<div>The C-terminal PBI of PCDH15-CD3 is necessary for its retention in the TGN of COS-7 cells by PIST. (a) In the presence of EGFP-PIST, Myc-PCDH15-CD3 colocalizes with EGFP-PIST in the TGN. (b) In the presence of EGFP-PIST, Myc-PCDH15-CD3 lacking the C-terminal PBI (MTKL) localizes in the cytoplasm as well as on the cell membrane. Myc-PCDH15 was stained with mouse anti-Myc antibody and then TRITC-conjugated goat anti-mouse secondary antibody. Nuclei were stained with DAPI. The fluorescent intensity was quantified using Image J. Scale bars, 10 <i>μ</i>m.</div>

Neural Plasticity

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Figure 4

Figure 4: Plasma Membrane Targeting of Protocadherin 15 Is Regulated by the Golgi-Associated Chaperone Protein PIST 