Plasma Membrane Targeting of Protocadherin 15 Is Regulated by the Golgi-Associated Chaperone Protein PIST

Figure 5

PIST reduces the membrane expression of PCDH15-CD3 in COS-7 cells. (a) Myc-PCDH15-CD3 immunoreactivity shows cytoplasmic and plasma membrane localization when coexpressed with EGFP. (a′) Myc-PCDH15-CD3 immunoreactivity using a nonpermeable protocol shows clear plasma membrane localization when coexpressed with EGFP. (b) Myc-PCDH15-CD3 immunoreactivity colocalizes with EGFP-PIST in the TGN. (b′) The plasma membrane localization of Myc-PCDH15-CD3 immunoreactivity examined using a nonpermeable protocol disappears when EGFP-PIST is present. For the nonpermeable protocol, staining was performed as normal except that Triton X-100 was excluded. Myc-PCDH15 was stained with mouse anti-Myc antibody and then TRITC-conjugated goat anti-mouse secondary antibody. Nuclei were stained with DAPI. Scale bar, 10 μm.